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. Author manuscript; available in PMC: 2013 Oct 28.
Published in final edited form as: Steroids. 2012 Apr 20;77(0):10.1016/j.steroids.2012.04.013. doi: 10.1016/j.steroids.2012.04.013

Fig. 2.

Fig. 2

Effect of DHT on COX-2 during IL-1β-stimulation is androgen receptor independent/ER-dependent in HBVSMC. Cyclooxygenase-2 (COX-2) protein expression was assessed in fetal human brain vascular smooth muscle cells (HBVSMC) pre-treated for 1 h with vehicle (VEH), the androgen receptor antagonist bicalutamide (BIC; 1 μM), or the non-selective estrogen receptor antagonist ICI 182,780 (ICI, 1 μM), followed by VEH or dihydrotestosterone (DHT, 10 nM) for 18 h, then exposed to interleukin-1 beta (IL-1β; 5 ng/ml; 6 h) in the continued presence of hormone/antagonist. (A) and (B) Representative blots for COX-2. C: Data analysis for COX-2 levels corrected for loading with beta actin and normalized to vehicle (VEH). *P < 0.01 vs. VEH, #P < 0.05 vs. IL-1β, #P < 0.05 vs. IL-1β, ˆP < 0.05 vs. IL-1β + DHT (n ≥ 4 per group).