Table 1.

Primer sequences used for quantitative real time reverse transcriptase polymerase chain reaction.

Target gene	Forward primer	Reverse primer	Product size
AR	5′-AGTGGATGGGCTGAAAAA-3′	5′-GGGTGTGGAAATAGATGGG-3′	305 bp
ER α	5′-AGGGAAAGTAGGGCAGAAA-3’	5′-ACGCTGGGAAATGAAGAA-3′	172 bp
ER β	5′-AAGAATATCTCTGTGTCAAGGCCATG-3′	5′-GGCAATCACCCAAACCAAAG-3′	143 bp
3α-HSD	5′-TGGGGTTGTGGTCCTGGCCA-3′	5′-CCACACGCAGGGCCTTCTGG-3′	229 bp
3β-HSD	5′-CAGCCAGGCATGGCCGACTC-3′	5′-CTGCTGCCACCTCATGGGCC-3′	235 bp
17βHSD	5′-AGAACCTGCTCTCCGCGCCT-3′	5′-TCCATTGGGGCCCCTCCTCC-3′	305 bp
CYP7B1	5′- ATGGCAGCAGTGCGTGACGA-3’	5′-CGCACACAGTAGTCCCCGGT-3′	221 bp
GAPDH	5′-GCCTTCTCCATGGTGGTGAA-3′	5′-GGTCGGAGTCAACGGATTT-3′	309 bp

Primer sequences used for qRT-PCR and product sizes in base pairs are listed for androgen receptor AR), estrogen receptors alpha (ERα) and beta (ERβ), 3β-hydroxysteroid dehydrogenase (HSD), 3β-hydroxysteroid dehydrogenase (3β-HSD), 3β - hydroxysteroid dehydrogenase (3α-HSD) and 17β -hydroxysteroid dehydrogenase (17β-HSD); CYP7B1 (3β-diol inactivation enzyme), and Glyceraldehyde 3-phosphate dehydrogenase (GAPDH).