Skip to main content
. Author manuscript; available in PMC: 2013 Oct 28.
Published in final edited form as: Ultrasound Med Biol. 2008 Apr 14;34(8):10.1016/j.ultrasmedbio.2008.01.005. doi: 10.1016/j.ultrasmedbio.2008.01.005

Fig. 4.

Fig. 4

Effect on air(4a) and calcein(4b) encapsulation of various solutes present during the freezing step, Liposomes were made by a procedure analogous to that described for gas-containing liposomes except that calcein was included (at 0.1 mM) in the each of the solutions of mannitol or glycine or PBS and added in the lipid hydration step. Liposome composition: DPPC:DOPC:CH 35:35:30. Under same treatment and pressure, mannitol, glycine and PBS solutions provided 38, 18 and 10 μl of gas encapsulation by 5 mg lipid and 22%, 23% and 17% calcein encapsulation efficency, respectively. Mean +/− SD, n=4. *p<0.05 vs trehalose group, ** p<0.01 vs all other groups.