Fig. 5.

Localization of SOCs to the basolateral side of polarized PDEC. Representative of 4 experiments. A differentiated PDEC monolayer were pretreated with 5 μM thapsigargin for 5 min in Ca²⁺-free Ringer’s. External 2 mM Ca²⁺-containing solution was applied to either luminal/apical or serosal/basolateral sides of PDEC. [Ca²⁺]_i is expressed as the fluorescence ratio at 340 and 380 nm.