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. Author manuscript; available in PMC: 2013 Nov 1.
Published in final edited form as: Nanomedicine (Lond). 2013 Mar 27;8(11):10.2217/nnm.12.204. doi: 10.2217/nnm.12.204

Figure 7. Confocal imaging of human mesenchymal stem cell protein localization and cell morphology following electrical stimulation.

Figure 7

(A) In the presence of ES, human mesenchymal stem cells cultured on poly(ε-caprolactone) substrates increased cell–cell contacts with neighboring cells and maintained strong expression of α-MYH as well as colocalization with F-actin. (B) In contrast, ES completely silenced expression of α-MYH for human mesenchymal stem cells on poly(ε-caprolactone) carbon nanotube substrates and caused cells to lose their elongated morphology. For cells cultured on either substrate, cotreatment with ES and Aza resulted in a complete deregulation of normal cell function as indicated by changes in cell morphology and either loss of α-MYH expression or colocalization with actin.

α-MYH: α-myosin heavy chain; Aza: Azacytidine; ES: Electrical stimulation; F-actin: Filamentous actin.