FIG. 6.

Equal prevention of renal iron deposition by Hp1-1 and Hp2-2. (A) Representative Perls iron stained kidney tissue with 3,3′-diaminobenzidine intensification showing nontreated (upper left), Hb (upper right), Hb+Hp1-1 (lower left), and Hb+Hp2-2 (lower right). (B) Image analysis was performed using ImageJ to determine the ratio of iron-stained area to the total area of 20 images (10×) of n=5 individual animals. Percentage areas are represented as mean±SE. (C) Ex vivo Hb binding of guinea pig plasmas was measured by SEC after infusion with either Hp1-1 or Hp2-2. The figure shows chromatogram areas (AU*min) of Hb-bound Hp components (=Hb:Hp complex) plotted against Hb concentration. Details are provided in Supplementary Figure S4. To see this illustration in color, the reader is referred to the web version of this article at www.liebertpub.com/ars