Figure S3.
Spatiotemporal Expression of Cnidarian Navs, Related to Figure 3
(A) Developmental time series of expression for the five Nav2 channel genes from N. vectensis. The data were generated from qPCR for five developmental stages. Expression is presented in molecules per μl cDNA. Bars represent mean + SE of n = 3 replicates.
(B–F) In situ hybridization with probes for NvNav2.1 (B), NvNav2.2 (C), NvNav2.3 (D), NvNav2.4 (E), and NvNav2.5 (F) revealed the spatial expression pattern of the different channel subtypes in 5-day-old N. vectensis larvae. A similar assay on C. hemisphaerica revealed the spatial expression patterns of two channel homologs.
(G–J) Probes for ChNav2.1 (G) and ChNav2.5 (H) in the gastrozoid developmental stage, and for ChNav2.1 (I) and ChNav2.5 (J) in the medusa stage. The oral end is indicated by an asterisk.