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. 2013 Oct 15;319(17):2728–2738. doi: 10.1016/j.yexcr.2013.07.029

Fig. 4.

Fig. 4

Regulation of BAX translocation to the mitochondria. Changes in the mitochondrial levels of BAX in NIH 3T3 and 32D cells exposed to GF abrogation were analyzed following the isolation of mitochondrial fractions of NIH 3T3 WT, (A) NIH gag-v-RAF (EHneo), (B) NIH 3T3 B-RAF V600E (V600E), (C) NIH 3T3 C-RAF S427G (S427G), (D) NIH 3T3 ERBXB cultured in the presence of 200 nM OHT (+/− OHT), (E) NIH 3T3 pBABE, NIH 3T3 pBABE AKTm/p (AKT) cells maintained in 10% or 0.05% FCS medium for 12 h and (F) 32D ERBXB cells (+/− OHT) cultivated in the presence or absence of IL-3 for 16 h were isolated and analyzed for BAX protein level. Examination of α-tubulin and VDAC served as a confirmation for purity of isolated mitochondria.