Fig. 5.

Effects of RAF on mitochondrial membrane potential. The effects of RAF signaling on the mitochondrial membrane potential following GF abrogation were studied in 32D cells expressing an OHT-regulated form of oncogenic RAF after IL-3 removal. (A) 32D ERBXB cells were cultivated in the medium containing 200 nM OHT (+OHT) or solvent (−OHT) in the presence or absence of IL-3 for 24 h and (B) 32D ERBXB cells (±OHT) incubated in IL-3 free medium containing DMSO, 20 µM BAY43-9006 (BAY), 25 µM UO126 (UO) or 25 µM LY294002 (LY) were subjected to mitochondrial membrane potential analysis with FACS. As a positive control for mitochondrial potential dissipation cells were treated with 5 µM FCCP. The graph depicts the percentage of cells gated with high mitochondrial potential (n=3, **p≤0.01, ***p≤0.001).