Fig. 6.

Regulation of intracellular signaling and cell survival by anti-oxidants. 32D cells were starved in the presence of antioxidant N-acetyl-cysteine (NAC). 32D WT cells were incubated in full growth medium, IL-3 free medium and in IL-3 free medium containing 20 mM NAC for 16 h, followed by (A) PARP and caspase-3 (casp3) cleavage, Mcl-1 and Bim protein expression, (B) BAX activation detection, (C) BAX mitochondrial protein level examination, (D) intracellular signaling pathways analysis (E) mitochondrial membrane potential was assessed in 32D ERBXB cultured in the presence of 200 nM OHT (+OHT) or its solvent (−OHT) and maintained for 24 h in media supplemented with IL-3, deprived of IL-3, or deprived of IL-3 containing 20 mM NAC (n=3, **p≤0.01).