Figure 11.

Preincubation with DCMU Affects Dark-Induced Transcript Accumulation.

C. reinhardtii wild-type CC-124 was grown under standard conditions in the light until a cell density of 3 × 10⁶ cells mL⁻¹. Then, the culture was split in two and 2.5 µM of the specific photosystem II inhibitor DCMU was added to one aliquot, while an equivalent volume of ethanol was added to the other. After further incubation in the light for 1 h, each cell suspension was transferred to either open beakers (+) or sealed flasks (–) in the dark for 0.5 h. RNA was isolated before the preculture was split (indicated by −1h), after 1-h incubation in the light with (dark-gray bars) or without (light-gray bars) DCMU (0h) and after 0.5 h of incubation in the dark (0.5h+ and 0.5h−). Transcript abundances were calculated relative to the RCK1 transcript (CBLP, Cre13.g599400). Values shown are averages of biological replicates. Error bars = sd. See Supplemental Figure 6C online for a scheme of the experimental setup.