Figure 9.

Impact of O₂ on HYDA1 Transcript in the Dark.

(A) Cells were incubated for the indicated time points in open (+O₂, light-gray bar) or sealed (−O₂, dark-gray bar) flasks in the dark. Cell concentrations at 0 h (white bar) were 2.2 × 10⁶ cells mL⁻¹.

(B) and (C) Light-grown cells (0 h, white bar) were incubated for 2.5 h in sealed flasks in the dark (dark-gray bar) and then transferred to open beakers in the dark (+O₂, light-gray arrow) or kept in sealed flasks (−O₂, dark-gray arrow) for the indicated time points. Cell numbers of the precultures were 2.7 (B) and 2.2 × 10⁶ cells mL⁻¹ (C).

RNA was isolated at the depicted time points. HYDA1 RNA hybridization was conducted with a HYDA1-specific probe. RCK1 (CBLP, Cre13.g599400) served as the reference transcript. Supplemental Figures 6A and 6B online provide schemes of the experimental setups.