Fv SOC1 Activates GA Biosynthesis to Promote Runner Growth in Strawberry.
(A) The effect of the inhibitor of GA biosynthesis, prohexadione-calcium (Pro-Ca), on runner formation in SD strawberry (VES) and Fv SOC1-OX lines. The cumulative number of runners (n/plant) is shown. Five-week-old plants were grown under LDs and treated with 100 ppm Pro-Ca or water (control) at week 0. Error bars represent se; n = 9 to 10.
(B) The effect of GA3 on runner formation in SD strawberry and Fv SOC1-RNAi lines. Cumulative number of runners is shown. Five-week-old plants were grown under LDs and treated with 50 ppm GA3 solution or mock treated (-GA) at weeks 0 and 2. Error bars represent se; n = 10 to 11; for RNAi #1, n = 5.
(C) and (D) Relative expression of Fv GA20ox
(C) and Fv GA3ox
(D) genes in the leaves of clonally propagated SD strawberry and Fv SOC1-OX line #7 grown under LDs. Mean fold change was calculated relative to the expression in SD strawberry separately for each gene. Error bars represent se; n = 2.
(E) and (F) Relative expression of Fv GA20ox
(E) and Fv GA3ox
(F) genes in the leaves of clonally propagated SD strawberry and Fv SOC1-RNAi line #1 grown under LDs. Mean fold change was calculated relative to the expression in SD strawberry separately for each gene. Error bars represent se; n = 3.