Figure 4.

Fni3 and Suv Catalyze Lys-63–Linked Ubiquitination.

(A) In vitro ubiquitination assay using GST-tagged Fni3, Suv, and At-PUB54 recombinant proteins. This experiment was repeated two times with similar results.

(B) Effect of Suv and Fen on Fni3-directed in vitro ubiquitination. One third and equal amount of Fen to AtPUB54 was added to the reactions of lane 5 and 6, respectively. This experiment was repeated three times with similar results.

(C) Fni3 and Suv catalyze Lys-63–specific ubiquitination. This experiment was repeated two times with similar results. The numbers on the right denote the molecular mass of marker proteins in kilodaltons.

[See online article for color version of this figure.]