Figure 9.
Fni3 Does Not Affect the Stability of Fen in Vivo.
Agrobacterium-mediated transient expression of HA-tagged Fni3, Fni3C89G, Suv, and Fen alone or coexpressed in the combinations as specified were performed on tomato RG-pto11 plants. The abundance of Fen, Fni3/Fni3C89G, and Suv were examined by immunoblotting using anti-HA antibody. Staining of ribulose-1,5-bis-phosphate carboxylase/oxygenase (Rubisco) subunits by Coomassie blue demonstrated equal loading. Asterisk denotes nonspecific hybridization band in the immunoblot. The numbers on the right denote the molecular mass of marker proteins in kilodaltons. This experiment was repeated two times with a similar result.
[See online article for color version of this figure.]