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. 2013 Oct 29;4(6):e00419-13. doi: 10.1128/mBio.00419-13

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Copyright © 2013 Li et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-ShareAlike 3.0 Unported license, which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original author and source are credited.

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FIG 1 — SuhB is required for the expression of the T3SS. (A) The expression of exoT-lacZ in the backgrounds of P. aeruginosa strains PAK, suhB::Tn5, suhB::Tn5 containing pUCP-suhB, ΔsuhB, and ΔsuhB containing pUCP-suhB. Bacteria were grown to an OD₆₀₀ of 1 to 2 in LB with or without EGTA before β-galactosidase assays. *, P < 0.05 compared to wild-type (WT) or complemented strains by Student’s t test. (B) Expression and secretion of ExoS in the indicated strains. (C) Cytotoxicity of the suhB mutant. A549 cells were infected with the indicated strains at a MOI of 20. At 4 h postinfection, cells attached to the plate were measured with crystal violet staining. *, P < 0.05 compared to WT or complemented strains by Student’s t test.