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. 2013 Oct 29;4(6):e00419-13. doi: 10.1128/mBio.00419-13

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Copyright © 2013 Li et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-ShareAlike 3.0 Unported license, which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original author and source are credited.

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FIG 3 — SuhB regulates the expression of exsA on the posttranscriptional level. (A) The expression of exsC-lacZ in indicated strains. *, P < 0.05 compared to WT or complemented strains by Student’s t test. (B) The construct of the Ptac-driven exsCEBA operon. (C) Relative mRNA levels of exsC, exsA, and the region between exsB and exsA under T3SS-inducing conditions. Bacteria were grown to an OD₆₀₀ of 1 in LB containing 1 mM IPTG and 5 mM EGTA before RNA purification. The mRNA levels were tested by real-time PCR. The locations of real-time PCR primers were indicated by arrows in panel B. (D) Expression and secretion of ExoS in indicated strains. (E) Constructs of exsA-FLAGs. (F) Expression of ExsA-FLAG and ExoS in indicated strains.