Table 1.
Composition of experimental diets.
| Composition (g/100g) | ||
|---|---|---|
| n-3 Adq. | n-3 Def. | |
| Casein, vitamin free | 20 | 20 |
| Carbohydrate: | 65 | 65 |
| Cornstarch | 10 | 10 |
| α-Cornstarch | 15 | 15 |
| Sucrose | 10 | 10 |
| Glucose | 20 | 20 |
| Dextrose | 5 | 5 |
| Maltose-dextrin | 5 | 5 |
| Cellulose | 5 | 5 |
| Mineral-salt mix | 3.5 | 3.5 |
| Vitamin mix | 1 | 1 |
| L-Cystine | 0.3 | 0.3 |
| Choline bitartrate | 0.25 | 0.25 |
| TBHQ | 0.001 | 0.001 |
| Fat: | 5 | 5 |
| Hydrogenated coconut oil | 3.875 | 4.05 |
| Saffower oil | 0.885 | 0.95 |
| Flaxseed oil | 0.24 | none |
| Fatty acid compositiona | ||
| Saturates | 75.1 | 78.1 |
| Monounsaturates | 5.4 | 4.7 |
| 18:2n-6 | 14.6 | 14.6 |
| 18:3n-3 | 2.53 | 0.25 |
| n-6/n-3 | 5.8 | 58.5 |
The two experimental diets, an n-3 fatty acid dequate diet (n-3 Adq) and an n-3 fatty acid deficient diet (n-3 Def), were based on the AIN-93 formulation with several modifications to obtain the extremely low basal level of n-3 fatty acid required in this study.
a
The 20:4n-6, 20:5n-3 and 22:6n-3 fatty acids were less than 0.01%, i.e., not detected (nd).