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Characteristics of thermal denaturation of FITC-BSA in the absence and presence of different honey concentrations as monitored by FITC fluorescence upon excitation at 495 nm.
| Protein sample |
Transition | ΔG D 25°C (cal mol−1) | ΔH (cal mol−1) | ||
|---|---|---|---|---|---|
| Start point (°C) | Midpoint (°C) | End point (°C) | |||
| FITC-BSA | 45.0 | 69.3 | 95.0 | 4400 | 41800 |
| FITC-BSA + 10% (w/v) honey | 57.5 | 75.0 | 95.0 | 5500 | 51000 |
| FITC-BSA + 20% (w/v) honey | 62.5 | 82.8 | 95.0 | 8400 | 55000 |
