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PCR Conditions for amplification of bla TEM, bla SHV, bla CTX-M genes bp: base pair
| Gene Detected | Denaturation Time/temp | Annealing Time/temp | Extension Time/temp | No. of cycles | Product |
|---|---|---|---|---|---|
| bla TEM | 94oC-1min | 58oC -1 ½ min | 72oC -1min | 30 | 1100bp |
| bla SHV | 94oC-1min | 58oC -1 ½ min | 72oC -1min | 30 | 930bp |
| bla CTX-M | 94oC-1min | 58oC -1 ½ min | 72oC -1min | 30 | 544bp |
