Table 1.

Orientation search and alignment between different parts of the 80S ribosome

	Initial alignment at 60S subunits	Alignment at complete 40S subunit	Alignment at 40S body/platform	Alignment at 40S head
	Angles:	Angles:	Angles:	Angles:
	α=0°	α=5°	α=5°	α=4°
	β=0°	β=−4°	β=−3°	β=−14°
	γ=0°	γ=1°	γ=1°	γ=7°
	Crosscorrelation coefficient
	Angles α/β/γ (in case of optimal alignment)
60S subunit	0.89	0.76	n.d.	n.d.
	0/0/0
40S subunit body/platform	0.83	0.92	0.93	0.59
			5/−3/1
40S subunit head	0.74	0.82	0.80	0.92
				4/−14/7
The corresponding ribosomal parts of the POST 80S ribosome (Spahn et al, 2001a) and of the 80S·eEF2·sordarin complex are compared in different orientations with the crosscorrelation coefficient. The relative rotational orientation is given in an orthogonal coordinate system, where the x-axis is parallel to the anticodon stem helix of the P-site bound tRNA, the y-axis is parallel to the acceptor stem helix, and the z-axis points from the A-site region toward the E-site region (see Spahn et al, 2001b). The cartoon shows the outline of the 40S subunit from the 60S site and the outline of the P-site bound tRNA, together with the axes representing the coordinate system. The angles α, β and γ describe rotations around the x-, y- and z-axis, respectively. n.d., not determined. Optimal alignment for a certain ribosomal region is indicated by bold numbers and by the angles in the table. The angles describe the rotational rearrangement from the conformation of the POST 80S ribosome to the conformation of the 80S·eEF2·sordarin complex.