Figure 1.

BCL-2^AAA-expressing cells are more resistant than BCL-2^wt-expressing cells to calcium-dependent death stimuli. (A) Jurkat cells expressing either neo vector, BCL-2^wt, or BCL-2^AAA were treated as indicated, stained with annexin V, and analyzed by FACS. (B) MEFs or (C) Jurkat lines were treated in medium without serum with 30 μM arachidonic acid for the indicated times. (D) Jurkat cells were treated in growth medium for 17 h with the indicated concentrations of H₂O₂. (E) Untreated Jurkat (left panel) or MEF (right panel) cells were fractionated as described in Materials and methods, and then run on SDS–PAGE and Western blotted with the indicated antibodies.