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Human dominant-negative mutant class II transactivator (CIITA-DN) construct. A 9·1 kb SalI fragment containing the human Tie-2 enhancer a dominant negative CIITA mutant cDNA driven by the cytomegalovirus enhancer/B-actin promoter (CAG), and an antibiotic resistance cassette, was used to transfect porcine fetal fibroblasts for subsequent nuclear transfer.
