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. 2013 Aug 12;140(1):39–46. doi: 10.1111/imm.12107

Figure 3.

Figure 3

Class II transactivator (CIITA) expression on dominant-negative CIITA (CIITA-DN) and wild-type (WT) porcine aortic endothelial cells (pAECs). Detection of mRNA levels for CIITA in quiescent and activated pAECs. CIITA-DN and WT pAECs were activated with porcine interferon-γ (pIFN-γ; 50 ng/ml) for 48 hr. (a) The expression of CIITA in pAECs was investigated by reverse transcription-PCR using specific primers for pCIITA. Significant levels of mRNA for CIITA were constitutively detected in quiescent WT pAECs, and were markedly increased in activated WT pAECs. In contrast, CIITA-DN mRNA was undetectable in pAECs when cells were quiescent, and minimally increased when cells were activated. (b) Quantitative real-time PCR showed a minimal increase of CIITA mRNA in CIITA-DN pAECs after activation with pIFN-γ (50 ng/ml) for 72 hr.