Figure 1.

Indomethacin and prostaglandin D₂ (PGD₂) induced chemotaxis in eosinophils through chemoattractant receptor-homologous molecule expressed on T helper type 2 cells (CRTH2) receptors. Cells were mixed with either indomethacin, PGD₂, or medium, and then applied to the upper chamber. Eosinophil migration to the lower chamber containing either indomethacin, PGD₂, or medium was assessed by photometric assay using eosinophil peroxidase activity (a, b). Eosinophil migration in response to indomethacin and PGD₂ (lower chambers) was assessed in the presence of CAY10471 (3 nm), a CRTH2 antagonist, in the upper chamber (c). Representative results of two independent experiments are shown.