Figure 4.

Chemoattractant receptor-homologous molecule expressed on T helper type 2 cells (CRTH2) -mediated eosinophil priming by Δ¹²-prostaglandin J₂ (PGJ₂) was cancelled by indomethacin. (a) Eosinophils were pre-treated with CAY10471 for 10 min at 37°. After centrifugation, they were mixed with Δ¹²-PGJ₂, then applied to the upper chamber. Chemotaxis to eotaxin (lower chamber) was assessed in the presence of Δ¹²-PGJ₂. Pre-treatment with CAY10471 cancelled the priming effects of Δ¹²-PGJ₂ on eosinophil migration to eotaxin. (b) Eosinophils were pre-treated with indomethacin for 90 min at 37°. After centrifugation, they were mixed with Δ¹²-PGJ₂, then applied to the upper chamber, and subjected to chemotactic assay for eotaxin. *P < 0·05. Representative results of at least three independent experiments are shown.