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. 2004 Feb 26;23(5):1020–1029. doi: 10.1038/sj.emboj.7600119

Table 1.

Mapping interactions between calnexin, calreticulin, ERp57, and PDI using MYTHS

  CNX H21P482 ERp57 A24-L505 PDI D18-L508 ERp57 K362R ERp57 F386S ERp57 W405R ERp57 F476S pLJ96 GST
ERp57 A24-L505 +++ + +++
CNX H21−P482 +++ +++ +++ +++ +++ +++
CNX P270−F415 (11112222) +++ +++ +++ +++ +++ +++
CNX D289−N393 (111222) +++ +++ +++ +++ +++ +++
CNX P310−P378 (1122) +++ +++ +++ +++ +++ +++
CRT E18-L417 +++ +++ +++ +++ +++ +++
CRT D201-A307 (111222) +++ +++ +++ +++ +++ +++
CRT A223-P283 (1122) +++ +++ +++ +++ +++ +++
PDI D18-L508 + n/a n/a n/a n/a +++
CNX2pmA + +++ +++ +++ +++ +++
CNX6pmA +++
pLJ89 +++
GST
+++
+++
+++
+++
+++
+++
+++
+++
+++
Results of using several calnexin and calreticulin P-domain constructs to map their interactions with ERp57. CNX and CRT deletion mutants are indicated, with the number of P-domain repeat motifs indicated in parenthesis. Specific point mutations in calnexin confirm acidic residues necessary for interaction. Error-prone PCR identifies point mutations in ERp57 that complement the mutated calnexin P-domain. The nature of each mutant fusion protein is described in the text. Plus symbols indicate the presence and strength of an interaction (‘+++' strongest to ‘+' weakest), and ‘−' indicates no interaction detected. ‘n/a' indicates data not available.