In vivo antitumor efficacy of CDDP-loaded nanogels and Pt accumulation in A2780 human ovarian cancer xenograft-bearing female nude mice. a) Tumor growth, b) body weight (calculated as body weight subtract tumor) and c) survival time after administration of 5% dextrose (1, control), CDDP alone (2), nanogel/CDDP (3), LHRH-nanogel/CDDP (4) at a dose of 4 mg CDDP equivalents/kg body weight every 4th day. d) Concentration of Pt in organs and tumor by ICP-MS. Mice were sacrificed in a week after receiving four i.v. injections of free CDDP, nanogel/CDDP and LHRH-nanogel/ CDDP. Data are mean ± SEM, n = 7-8, *p < 0.05, **p < 0.01, NS is not significant.