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. Author manuscript; available in PMC: 2014 Sep 1.
Published in final edited form as: Mol Cell Neurosci. 2013 Jul 31;56:10.1016/j.mcn.2013.07.010. doi: 10.1016/j.mcn.2013.07.010

Figure 3. Darkness reduces the interaction of GRIP with GluA2.

Figure 3

A. The interaction of GRIP with GluA2 was measured in retinal lysates from rats housed in light (Light) or darkness (Dark) for >8 hours. GluA2 and bound proteins were immunoprecipitated and separated by SDS PAGE. Western blots were probed for precipitated GluA2 and bound GRIP. Total lysates from which proteins were precipitated were also probed for GluR2 and PSD-95 levels. B. Quantitation of experiments as shown in A. GRIP-bound GluA2 intensities are normalized to mean values of the light exposed samples. The proportion of GRIP bound GluA2 decreases significantly in retina of animals maintained in darkness for 12 hours (Dark) compared to those housed in light (Light) (n=5, * p< 0.05).