Figure 8. Proteasome-dependent cross-priming confirms the significance of the regulatory mechanism.

(A) B78OVA cells were preincubated with 50 μM lactacystin or DMSO at 37°C for 2 hours. Cells were washed and subjected to 3 FT cycles. SF from FT cells was collected and heat inactivated. Titrated amounts of LC-treated or control SF was cultured in vitro with purified DCs and T cells, and T cell activation was analyzed. Data are representative of 3 independent experiments. (B) B78OVA cells were preincubated with 100 μM lactacystin or DMSO at 37°C for 2 hours. After washing, FT, and subsequent heating, 2 × 10⁶ cells were injected in mice on days 0 and 2, and Ag-specific immune responses were analyzed by in vivo CTL assay on day 7. Data are representative of 2 independent experiments. (C) Potential mechanism of immune response generation during sterile necrotic cell death and control of cross-priming by cellular peptidases. *P < 0.05, ***P < 0.001, 1-way ANOVA with Bonferroni’s paired-comparison test (A) or Student’s t test (B).