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. 2013 Oct 25;123(11):4923–4934. doi: 10.1172/JCI69355

Figure 8. Itch-deficient Treg cells instruct the differentiation of naive T cells into Th2 in vitro.

Figure 8

(A–C) FACS-sorted CD62Lhi CD44lo naive CD4+ T cells were cultured with supernatants (sup) from wild-type or Itch-deficient Treg cells for 5 days. Anti–IL-4 antibodies were added where indicated. (A) IL-4– and IFN-γ–producing cells were analyzed by intracellular cytokine staining 5 hours after restimulation with anti-CD3/CD28 antibodies. Data are representative of three independent experiments. (B and C) RT-PCR analysis of the indicated genes in these cells is shown. Data are compiled from three independent experiments. Error bars indicate the mean ± SD. **P < 0.01; ***P < 0.001 (unpaired 2-tailed Student’s t test).