Figure 7.

Expression of myr-Cdc24p lacking its carboxy-terminal PB1^Cdc24-domain is toxic. (A, B) Schematic representation of the Cdc24p-deletion constructs that were fused to the myristoylation signal (A). The expression levels of the indicated constructs were examined in wild-type cells by immunoblotting with Cdc24p- (B, left blot) or GFP-antibodies (B, right blot). (C) Wild-type (K699) cells overexpressing either no protein (vector), myr-Cdc24p or the indicated myr-Cdc24-mutant proteins from the GALL-promoter were photographed after 3 days at 30°C on plates containing glucose (left plate) or galactose (right plate).