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. 2013 Oct 15;123(11):4769–4780. doi: 10.1172/JCI70528

Figure 3. Ectopic expression of itch mediators in BRAFNav1.8 mice.

Figure 3

(A–I) BRAFNav1.8 mice showed ectopic expression of (A–C) GRP, (D–F) Mrgpra3, and (G–I) H1R in DRGs. (J–R) BRAFNav1.8 mice showed increased expression of (M–O) Mrgpra3 (blue) but not (J–L) GRP (brown) and (P–R) H1R (brown) in Trpv1+ cells (blue in J–L and P–R; brown in M–O). (S–X) Enhanced expression of GRPR as shown by (S–U) GFP staining and (V–X) Grpr mRNA in the dorsal horns of BRAFNav1.8 mice. (Y–AA) Enhanced GRP in primary afferents of BRAFNav1.8 mice. All mice were 8~12 weeks of age, except in V–AA, where 2-week-old mice were used. Scale bar: 20 μm. (J–Q) Arrows and arrowheads indicate TRPV1+ and TRPV1– neurons, respectively. *P < 0.05, **P < 0.01, ***P < 0.001, BRAFNav1.8 mice vs. wild-type. n = 4.