Figure 3.

When activated by Src64, Tec29 can generate docking sites for itself. (A–H) ΔKinase localization to ring canals was used as a reporter of available Tec29 docking sites in various backgrounds. (A) wild-type; (B) Tec^l(2)k05610 mutant; (C) Src64^Δ17 mutant; (D) wild-type background with coexpression of a type 1 transgene; (E) Src64^Δ17 mutant background with a type 1 transgene; (F) Tec^l(2)k05610 mutant background with a type 1 transgene; (G) wild-type background with coexpression of a kinase-inactive (K356M) type 1 transgene; (H) Src64^Δ17 mutant background with coexpression of a kinase-inactive (K356M) type 1 transgene. The same ΔKinase transgene on the second chromosome was used for all except (C) and (F), in which a third chromosome transgene insert that expresses comparable levels of Δkinase was used. (I) Kinase-inactive type 1 Tec29 failed to localize to ring canals in a Tec^l(2)k05610 mutant background.