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. 2004 Feb 19;23(5):1089–1100. doi: 10.1038/sj.emboj.7600127

Figure 4.

Figure 4

Both types of Tec29 are phosphorylated in an Src64-dependent manner. (A–F) Src64 kinase activity is required for Tec29 localization and ring canal growth. (A, C, E) Immunostain with anti-Tec29. (B, D, F) Immunostain with anti-HTS. HTS is the Drosophila homolog of adducin and a ring canal component (Yue and Spradling, 1992). (G) Average ring canal size of Src64Δ17/Src64Δ17 and [Src64KD]/+; Src64Δ17/Src64Δ17 ovaries. Images of 10 stage-10A egg chambers stained with anti-HTS (such as the ones in (D) and (E)) were taken and ring canals sizes were measured according to Materials and methods. (H) Size distribution of ring canals in (G). (I) Both types of Tec29 are phosphorylated in an Src64-dependent manner. GFP-tagged type 1 (left panels) or type 2 (right panels) Tec29 was immunoprecipitated from a wild-type or Src64Δ17/Src64Δ17 background and analyzed by Western blotting and probing with anti-pY (bottom panels), then stripped and reprobed with anti-Tec29 antibodies (top panels).