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Tiam1 binds to ephrin-B1 and EphA2 in vitro: [35S]methionine -labeled wild type or truncated mutants of Tiam1 (C1199, N1041, N392, PHnTSS) translated products were incubated with glutathione–agarose-conjugated GST, GST-ephrin-B1264−346 and GST-EphA2563−977, respectively. IVT, the input of in vitro translation reaction before the beads binding.
