Figure 6.

EphB2-Fc induces neurite outgrowth of E14 mouse cortical neurons. Primary cultured cortical neurons from E14 mouse cerebral cortex were seeded on plates coated with EphB2-Fc (A), Fc fragment (B), bovine serum albumin (BSA) (C) or ephrin-A1-Fc (D), as described in Materials and methods (Neurite elongation assay). Representative pictures are shown after incubation for 20 h (A–D). (E) Expression of Tiam1, ephrin-B1 and various Eph receptors in dissociated primary cultured cells after 20 h incubation on poly-L-lysine-coated dishes, or in the cortical tissues was monitored by immunoblotting. (E, bottom) E14 primary precursors plated on an EphB2-Fc-coated dish were lysed for the indicated period, and immunoprecipitated with anti-ephrin-B1. Tyrosine phosphorylation of ephrin-B1 is shown by immunoblotting.