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. Author manuscript; available in PMC: 2014 Aug 1.
Published in final edited form as: Eur J Immunol. 2013 Jun 21;43(8):10.1002/eji.201343433. doi: 10.1002/eji.201343433

Figure 1. Specific recognition of CD300a by newly generated hybridomas.

Figure 1

(A, B) FACS analysis of (A) BW cells, BW expressing CD300c, YTS ECO cells and YTS ECO expressing CD300a cells and of (B) bulk NK-cell cultures derived from two different donors. The various hybridomas are indicated above the histograms. The anti-CD300a/c antibody recognizes both CD300a and CD300c. Staining is indicated by black-line histogram and background (filled gray histogram) is the staining with allophycocyanin-conjugated F(ab')2 goat anti-mouse IgG only. The gating strategy is shown in the left part of the figure. Data shown are representative of one out of three independent experiments performed.