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. Author manuscript; available in PMC: 2014 Aug 1.
Published in final edited form as: Eur J Immunol. 2013 Jun 21;43(8):10.1002/eji.201343433. doi: 10.1002/eji.201343433

Figure 6. CD300a and CD300c interact with PS expressed by tumor linese.

Figure 6

(A) The indicated tumor cells were double stained with PI and PS (left). Values in quadrants indicate percentages. FACS analysis of the indicated different tumor cell lines harvested with or without trypsin (black and gray open histogram respectively) and stained with anti-PS mAb (right histograms). Background (filled gray histogram) is the staining with allophycocyanin-conjugated F(ab')2 goat anti-mouse IgG only. (B) The indicated tumor cells were double stained with PI and with AnnexinV (left). Values in quadrants indicate percentages. FACS analysis of the indicated tumor cell lines harvested with or without trypsin (black and gray open histogram respectively) and stained with Annexin V (right). (C, D) The various tumor cells indicated in the figure were double stained with anti-PS (C) or with PI (D) together with the indicated fusion proteins. The numbers in the figure indicate percentages. (E) The various tumor cells (each tumor cell line is represented by two histogram rows) were treated without trypsin (-, upper histogram row in each tumor cell line) or with Trypsin (+ lower histogram row in each tumor cell line) incubated with or without 0.5 μg MGF-E8 (black and gray open histogram respectively) and then incubated with the indicated fusion protein. Data shown are representative of one out of three independent experiments performed.