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. Author manuscript; available in PMC: 2014 Sep 18.
Published in final edited form as: Neuron. 2013 Sep 18;79(6):10.1016/j.neuron.2013.07.037. doi: 10.1016/j.neuron.2013.07.037

Figure 3. In vitro and ex vivo labeling of NFTs in PS19 mice with PBB compounds.

Figure 3

(A) Double fluorescence staining of intraneuronal tau aggregates in postmortem brain stem slices of a 12-month-old PS19 mouse with PBB, other amyloid ligands and anti-phospho-tau antibody (AT8). (B) Binding of intravenously administered PBBs (0.1 mg/kg PBB5 and 1 mg/kg PBB1 and PBB3) to NFTs in PS19 mice at 10–12 months of age. The tissues were sampled at 60 min after tracer administration. The brain stem (top row) and spinal cord (second and third rows from the top) sections abundantly contained neurons showing strong fluorescence (left), and subsequent staining with FSB or AT8 (right) indicated that these cells were laden with tau amyloid fibrils (right). Putative intraneuronal tau inclusions in unsectioned spinal cords (arrowheads in the bottom row) removed from PS19 mice at 60 min after intravenous injection of PBB2 and PBB4 were also clearly visible by using a two-photon (2P) fluorescence microscopic system. Arrow in the bottom row indicates a cluster of autofluorescence signals from blood cells. Scale bars: 25 μm (A); 30 μm (top to third rows in B); 20 μm (bottom row in B).