Fig. 6.

Detection of ER stress (UPR) associated genes. (A) Detection of spliced XBP1 protein, TRAF2 protein, e-IF-2α protein. (B) Detection of spliced XBP1 protein. (C) Detection of TRAF2 protein. (D) Detection of eIF-2α protein. The relative values of spliced XBP1, TRAF2 and eIF-2α proteins were evaluated by the calculation of gray scan value of each preparation vs. that of β-actin protein. The mean data of each preparation were the results of three independent western blots scan and calculation, and indicated as mean±SD. Statistical differences of the data of cisplatin treatment compared with that of non-treatment are illustrated as ^*p<0.001 respectively. ER, endoplasmic reticulum; UPR, unfolded protein response; XBP1, X-box transcription factor-1; eIF-2α, eukaryotic translation initiation factor-2α; siLPT, siRNA against leptin; TRAF2, tumor necrosis factor receptor-associated factor 2.