Figure 4.

A DN inhibitor of Src tyrosine kinase relieves YAP-mediated suppression of Runx2 activity. ROS 17/2.8 cells were transfected with 1 μg rOC −208/CAT reporter along with 250 ng of Runx2 and/or YAP expression constructs. Two different concentrations of Src DN construct (200 and 800 ng) were used (bars 2 and 3, respectively, of each group). Cells were harvested 24–30 h after transfection and were subjected to CAT reporter assay. The graph represents results obtained from three independent experiments (n=6 each).