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. 2004 Feb 12;23(4):790–799. doi: 10.1038/sj.emboj.7600073

Figure 6.

Figure 6

Inhibition of endogenous Src signaling or YAP activity induces endogenous osteocalcin gene transcription. (A) Total cellular RNA from ROS 17/2.8 cells was analyzed by northern blotting and treated with indicated concentrations of PP2 for 1 h. Endogenous osteocalcin gene transcription is induced in a dose-dependent manner when the activity of Src family members is inhibited by PP2 (upper panel). 18S RNA was used as a loading control (bottom panel). (B) Activity of Src tyrosine kinase or YAP was selectively inhibited by overexpressing DN inhibitors (Src DN for Src tyrosine kinase and YAP DN for YAP). Total cellular RNA was subjected to northern blot analysis from ROS 17/2.8 cells overexpressing the indicated plasmids. As shown in the top panel, the DN inhibitor of Src (lane 2) or YAP (lane 4) induces endogenous osteocalcin gene transcription. (C) Densitometric analysis reveals the extent to which endogenous osteocalcin is induced under each experimental condition analyzed. (The bar graph represents the ratio of densitometric units of osteocalcin and 18S transcripts.)