Table 1.

Species-specific primer sets with optimum PCR cycles are shown in this table. All primer sets demonstrate specificity at 65 °C annealing temperature, a full description of the touchdown PCR cycle is available in the Materials and Methods section. Gel electrophoresis of the PCR products reveals visible bands at the expected amplicon lengths (Fig 4)

Species-specificity	Primer set name	Amplicon length	Forward primer 5′–3′	Reverse primer 5′–3′	Number of cycles
P. chrysogenum	benA_Chrysogenum	111	CATGTGAGTACAATGACTGGGAATCTT	TCGACCAGAACGGCACG	35
P. rubens	crt1_Rubens	180	CCTCGAGGACTTCAATCCTCCCGTG	GTCAGCGGGCTGAGTGGCC	28
P. chainii	crt1_Chainii	185	CTTTCTACAATTGCTCGCGTTTTTATTTG	CCTTGTTAGTGGCACCGCACTTA	35
P. floreyi	parA_Floreyii	111	ACGGCCCCTCCTTACGAAA	TGTGAGACCAAAGGCAGTGG	32