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. 2004 Feb 5;23(4):823–832. doi: 10.1038/sj.emboj.7600079

Figure 2.

Figure 2

DCX and JNK interact. (A–C) DCX and JNK co-immunoprecipitated from transfected cells. Cells were transfected with FLAG-tagged DCX, myc-tagged JNK2–MKK7 kinase dead or active as indicated. In each of the blots, the left lane is the extract, the middle lane is immunoprecipitations from cells transfected with one plasmid, and the right lane is the test immunoprecipitations. (A, C) Immunoprecipitations with anti-FLAG antibodies, immunoblot with anti-myc antibodies. (B) Immunoprecipitations with anti-myc antibodies, immunoblot with anti-FLAG antibodies. (D) Pull down experiments from transfected cells. Cells transfected with myc–JNK2–MKK7 kinase active were subject to GST pulldown using the following GST fusion proteins (order left to right): GST, DCX, pep1 (amino acids 51–135), pep2 (amino acids 178–259), pep1+2 (amino acids 51–259), Cter (from amino acid 273 to end). Note that both pep1 and pep2 interacted independently with myc–JNK2–MKK7. (E) Pull down experiments from P7 mouse brain extracts. The following were the recombinant proteins (order left to right): GST, GST–DCX, GST–pep1, GST–pep2, GST–pep1+2, GST–Cter. The GST proteins were checked by immunoblot (lower blots in D, E).