Localization of DCX and the JNK signaling module molecules in rat primary hippocampal neurons. (A–C) Colocalization of DCX and JIP-1 in some primary hippocampal neurons. DCX stained in green; some of the tips are marked with arrowheads (A), JIP in red (B) and some of the overlapping dots are indicated (arrowheads) (C). (D–F) Colocalization of p-DCX (D) and conventional kinesin (E), overlap in (F). (G–I) Colocalization of p-DCX (G) and p-JNK (H), merge in (I), in primary hippocampal neurons pretreated with kainic acid. (J–L) DCX–DsRED and JIP-1 colocalized in transfected neurons: DCX–DsRED (J) myc-tagged-JIP-1 stained with anti-myc antibodies (in green, K), merge (L), note the labeled tips of the neurites (small arrows, L).