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. 2013 Apr 15;2(Spec Iss):S0001. doi: 10.5702/massspectrometry.S0001

Fig. 7. Top: General reaction scheme depicting the Mannich reaction. Bottom: Nanospray-MS of a reaction mixture of 33 mM piperidine and 28 mM acrylamide after 1 h bulk solution-phase reaction (left), and 10 min surface reaction (right). In both cases 2 μL of each reagent in acetonitrile was used without catalyst. After 10 min reaction time, the surface reaction mixture was dissolved in 10 μL of methanol–water (1 : 1, vol/vol) and analyzed using nanospray ionization (1.8 kV; left spectrum). Similarly, after 1 h bulk-phase reaction, 10 μL of methanol–water (1 : 1, vol/vol) was added to the reaction mixture for MS analysis (right spectrum). Adapted from ref. 69 with permission. © 2012 Springer Science+Business Media.

Fig. 7. Top: General reaction scheme depicting the Mannich reaction. Bottom: Nanospray-MS of a reaction mixture of 33 mM piperidine and 28 mM acrylamide after 1 h bulk solution-phase reaction (left), and 10 min surface reaction (right). In both cases 2 μL of each reagent in acetonitrile was used without catalyst. After 10 min reaction time, the surface reaction mixture was dissolved in 10 μL of methanol–water (1 : 1, vol/vol) and analyzed using nanospray ionization (1.8 kV; left spectrum). Similarly, after 1 h bulk-phase reaction, 10 μL of methanol–water (1 : 1, vol/vol) was added to the reaction mixture for MS analysis (right spectrum). Adapted from ref. 69 with permission. © 2012 Springer Science+Business Media.