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. Author manuscript; available in PMC: 2013 Oct 28.
Published in final edited form as: J Thromb Haemost. 2012 Jul;10(7):10.1111/j.1538-7836.2012.04746.x. doi: 10.1111/j.1538-7836.2012.04746.x

Figure 3. Comparison of 1399R and 1399H rVWF collagen binding.

Figure 3

Recombinant full-length VWF was expressed in HEK293T cells using 100% wild-type DNA (1399R, shown in black), 100% variant DNA (1399H, shown in white), or a mixture of 50% of each DNA (1399R/H, shown in gray). Supernatants were analyzed for VWF:Ag and VWF:CB with types I, III, and VI collagen. Graphed here is the ratio of VWF:CB/VWF:Ag for each construct with each type of collagen. Data represent a minimum of 3 separate transfections, and are given as the mean + 1 SD.