Effects of long-term hyperglucagonaemia induced by infusion of glucagon via osmotic minipump (1 μg/h, i.p., for 4 weeks) and concurrent blockade of GCGR by [des-His1-Glu9] glucagon (5 μg/h via osmotic minipump, i.p.) on fasting blood glucose (A) and glucose tolerance (B) in glucagon-infused mice. **p<0.01 compared with basal (week 0) for fasting blood glucose (in A) or compared with 0 min for the GTT within the same group (in B). ++p<0.01, compared with glucagon-infused mice at corresponding periods. Ant, [Des-His1-Glu9]glucagon. Reprinted with permission from Li et al. [14]. Long-term hyperglucagonaemia induces early metabolic and renal phenotypes of Type 2 diabetes in mice. Clin Sci (Lond) 2008; 114(9):591–601.