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2,AP fluorescence changes in 106-basepair DNA templates containing 2,AP at the −2 position of the galP1 promoter as a function of time upon manual mixing with RNA polymerase in a Perkin-Elmer spectrofluorometer, and expressed as a ratio of two channels. The 2,AP fluorescence increased with time when the promoter was galP1+ (filled circles), and decreased initially and reached a plateau when the promoter was galP1− (open circles).
